Fluorescent Molecules and Dyes
Fluorescent molecules, also called fluorophores or simply flour’s, respond distinctly to light compared to other molecules. As shown below, a photon of excitation light is absorbed by an electron of a fluorescent particle, which raises the energy level of the electron to an excited state. Fluorescent dyes are non-protein molecules. They are often used in the fluorescent labeling of biomolecules and can be smaller or more photo stable than fluorescent proteins but cannot be genetically encoded.
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